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Following decalcification, this half was embedded in paraffin, sectioned at 5 m using a microtome (Leica RM2235, Nussloch, Germany), and stained with haematoxylin and eosin (H&E) following deparaffinisation procedures (xylene washes and graded alcohol dehydration/rehydration)

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Total RNA was extracted according to the instructions for using the Trizol kit (ThermoFisher Scientific, Shanghai, China) and reverse transcribed into complementary DNA cDNA at 37C for 15 min and at 85C for 5 s

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Cu hi 11: C cn phi trnh nhng loi thc phm no khi s dng Glutathione 600 khng