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Dongiovanni P, Fracanzani AL, Fargion S, Valenti L

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After release of the protoplasts with a pipette, the suspension was transferred to a filter-sterilized MSC solution (10 mM MES pH 5.8, 0.4 M sucrose, 20 mM MgCl 2 .6H 2 O, 467 mM mannitol) and overlaid with MMM solution

Concurrently, the levels of LH, E2, and FSH in the HEP treatment group were markedly elevated compared with the control group, suggesting that HEPs substantially increase serum reproductive hormone concentrations [58]