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Description
Do not shake, vortex, or invert vigorously mechanical agitation can denature peptide secondary structure
however, the clinical significance and durability of these effects remain under investigation

Galectin-3: a novel antiapoptotic molecule with a functional BH1 (NWGR) domain of Bcl-2 family
This is thought to be secondary to the cells ability to lie dormant, increase DNA repair and drug efflux capacity, and decrease apoptosis
Pediatr Radiol 1997;27:1558