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The blots were probed with the primary antibodies specific for phosphorylated PERK and CHOP (Thermo Fischer Scientific Inc.) at 4C overnight, then the blots were rinsed 35 times for 5 min with TBST and incubated with the horseradish peroxidase (HRP)-conjugated anti-rabbit IgG Secondary Antibody (Thermo Fischer Scientific Inc.) against the blotted target protein for 1 h at room temperature

HUVECs were treated with 1.0 g/ml BPC 157 and the protein expressions of Src, Cav-1, eNOS and their phosphorylated forms were analyzed by western blot

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These findings suggest that administering hepsin can effectively regulate GJ expression in wild-type mice, highlighting its potential as a therapeutic target to protect against APAP-induced hepatotoxicity, potentially in human patients