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To dissociate biotinylated proteins from streptavidin, boil the beads in 0.1% SDS or SDS-PAGE buffer for 3 mins or incubate them in 8 M guanidinium hydrochloride, pH 1.5
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In addition, using fly AD models, it was reported that an increase in H3K27ac and H3K9ac accelerates amyloid-42 (A42)-induced neurodegeneration in vivo
Statistical analyses were performed by unpaired two-tailed Students t test ( e )
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