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They can also inhibit the expression of Claudin-5 protein, widen the gaps between tightly connected endothelial cells, and increase BBB permeability
Transfection of overexpressing or siRNA plasmids were carried out in 6070% confluent cells by using Lipofectamine 3000 reagent according to the manufacturers protocol

Membranes were washed three times for 10 min each in TBST, then imaged on an LI-COR Odyssey CLx imager
By integrating omics strategies, bioinformatics analysis, NP, and MD simulation, researchers can systematically reveal the molecular mechanisms of cancer occurrence and development

Lepidium meyenii (maca) improved semen parameters in adult men
