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[DOI] [PubMed] [Google Scholar] 177.Nimmo MA, Leggate M, Viana JL, King JA
Option 4: PBS (pH 7.4) for Direct Assay Use For in vitro receptor assays where the working concentration is in the nanomolar range, phosphate-buffered saline at physiological pH is a practical choice
Interestingly, the significant increase observed in gh and pit-1 expression when GH3 cells were incubated in the presence of ghrelin was abrogated when these cells were pre-incubated or co-incubated with NESF or NLP
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