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For the reduction reaction, 300 L of freshly prepared 64 mM DTT/25 mM NH 4 HCO 3 was added, followed by incubation at 56C for 1 h with gentle agitation

Simplifying and Streamlining Escherichia Coli-Based Cell-Free Protein Synthesis

Folate, vitamin B6, and choline play essential roles in methylation and homocysteine regulation

Moreover, thioredoxin reductase purified from human T-cells plays an important role in reducing tumor cell growth [95]
