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Hair pigmentation is regulated by melanogenesis within the follicle and modulated by various factors, including melanocyte-stimulating hormone (MSH), adrenocorticotropic hormone (ACTH), endothelin-1, prostaglandins, leukotrienes, fibroblast growth factors, nitric oxide, catecholamines, as well as essential vitamins and minerals

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In 2 mL culture in glass tubes, a range of antibiotic concentrations was added (in technical duplicate) and the cells were grown for 8 h (~1012 generations), again at 37 C with 230 rpm shaking

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