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The membranes were then incubated with horseradish peroxidase-linked secondary antibodies (Cell Signaling Technology) and the signals were visualized by enhanced chemiluminescence using an Amersham Imager 600 (GE Healthcare)
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These effects collectively contribute to the immunomodulatory properties of Cbl, which raises the possibility that it could affect how the immune system reacts to autoimmune diseases such as MS [30,31,32]

Aschner M
Effects of water-pipe smoking on lung function: a systematic review and meta-analysis

Allergen-induced increases in IL-5 receptor -subunit expression on bone marrow-derived CD34 + cells from asthmatic subjects