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The multiplex PCR was performed under the following conditions: 5 min of initial denaturation at 94, followed by 35 cycles of 1 min at 94, 30 sec at 56, 45 sec at 72, and a final extension for 5 min at 72

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Several animal studies have reported that huperzine A may result in a more prolonged increase in acetylcholine in the whole brain when compared to other compounds that raise acetylcholine levels, such as tacrine , physostigmine , and metrifonate [5, 6, 7]

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N-Acetylcysteine (NAC): Helps reduce inflammation that can occur when the body is stressed by lack of fluids