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First of all, there may be difference in the intensity of ROS formation by paraquat and/or in ROS suppression by antioxidant, between in vitro cell line and cells in physiologic state

While Lip-1 alleviated the lethality of DEM (Figure 1E), it did not prevent the fall in glutathione that was induced by aging (as assayed on Day 4) or by DEM (Figure 1F)

[PMC free article: PMC4878518] [PubMed: 27140638] 263

Fisetin, a potential caloric restriction mimetic, attenuates senescence biomarkers in rat erythrocytes

Next, the cells were pelleted by centrifugation at 4,500 g for 10 min at 4 C (high-speed centrifuge CR22N, Eppendorf)