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TB-500 protocol considerations Reported dosing in the research literature: Starting dose: 500 mcg daily, subcutaneous injection Titration: Increase by 100 to 150 mcg every two weeks as tolerated Target dose: 750 to 1000 mcg daily by weeks five through twelve Weekly total: Approximately 5 mg per week at full dose Cycle length: Eight to twelve weeks, with optional extension to sixteen weeks TB-500 is typically administered once daily via subcutaneous injection, with consistent timing and systematic site rotation

doi: 10.4103/ijo.IJO_1161_20 187 Al AmryMAl GhadeerHAl GethamiAR

Gene therapy using CRISPR-Cas9 and AAV-mediated wild-type OPA1 expression is in preclinical development [178], with CRISPR-based gene editing proven successful in vitro for the OPA1 missense mutation [179]