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Description
Reduction and alkylation of the cysteine residues were then performed with 10 mM DTT and 55 mM iodoacetamide, respectively, followed by adding 360 L of 50 mM ammonium bicarbonate buffer to reduce the urea concentration to below 1 M
doi: 10.1136/bmj.307.6898.264
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doi: 10.1016/s0939-6411(00)00090-4
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