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It is obtained from your diet and vegans are more likely to have a deficiency

The 50 mg vial is reconstituted with 2 mL of bacteriostatic water to achieve a 25 mg/mL concentration (0.25 mg per unit)

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But at higher concentrations like 20mg/mL, 1 unit of dead space equals 200mcg, and 5 draws waste 1,000mcg (1mg), which is 5% of a 20mg vial