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The supernatant was incubated with anti-Flag M2 affinity gel (Sigma), and bound protein was eluted with 100 g ml 1 Flag peptide

Pro-inflammatory adipokines (e.g., leptin, resistin) and adipose-derived exosomes (carrying miRNAs like miR-3074-5p) create a chronic inflammatory joint microenvironment by polarizing synovial macrophages toward an M1 phenotype and inhibiting anti-inflammatory adiponectin (1618)

Matrix Derivatization The rational design of novel MALDI matrices provides a compelling approach to achieving specific physicochemical properties by strategically modifying the structures of classical matrices

Targeting mitochondria in Alzheimer disease: rationale and perspectives

NAD+ (Nicotinamide Adenine Dinucleotide) is a vital coenzyme found in every cell of the body, playing a key role in energy production, DNA repair, and cellular function