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Nitric oxide activates superoxide dismutase and ascorbate peroxidase to repress the cell death induced by wounding

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10.1016/j.devcel.2015.01.031 47 WangX.LiuY.HeJ.WangJ.ChenX.YangR

In contrast, the VPA-exposed group showed substantial pathological alterations, including mitochondrial pathologies featuring cristae disorganization and membrane rupture with alternating swelling and shrinkage (Fig

However, since cell counting is only a rough proxy to assess cytotoxicity and a higher dose escalation is necessary for CC 50 determination, we further included careful assessment of growth kinetics by live cell imaging, measurement of mitochondrial metabolic activity by MTT assay as well as cellular ATP levels by CellTiter-Glo (CTG, see Material and Methods section for details)