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Conventional oral glutathione is largely degraded in the stomach and intestine, leading to poor systemic and intracellular absorption

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The section was then incubated with recombinant anti-mucin 5AC antibody (ab3649, Abcam) for 1 h at room temperature, followed by biotinylated secondary antibody (Dako) and HRP detection (HRP conjugated ABC system, Vector Laboratories), according to manufacturers instruction