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This elevation in NAD+ activates sirtuin-1 (SIRT1), often referred to as the longevity gene, which plays a vital role in DNA repair, inflammation reduction, and metabolic regulation

doi: 10.1038/s41380-023-02330-6
doi: 10.1053/j.gastro.2009.06.049

Western Blot Western blotting was carried out as described previously [21,22], and brain homogenates were lysed on ice for 30 min in 100 mL of lysis buffer (120 mM NaCl, 40 mM Tris (pH 8), and 0.1% NP40) and centrifuged at 12,000 rpm for 30 min

Oseguera-Lopez I, Ruiz-Diaz S, Ramos-Ibeas P, Perez-Cerezales S
