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Coles et al
For analysis of PVMs, 40 m floating sections were permeabilized for 15 min with 1 PBST, and blocked in blocking buffer for 1 h
Furthermore, the DTI metrics that are used as proxies of myelin integrity do not specifically identify myelin abnormalities, but detect differences in water diffusiona parameter that can be altered by axon packing density, axon caliber, and more confounding factors (Chang et al., 2017)
Microbiome as an immune regulator in health, disease, and therapeutics
doi: 10.1111/j.1582-4934.2008.00163.x
