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demonstrated that, transfusion of RBCs stored for longer than 4 wk, considerably increased plasma free Hb.34 Additionally, a study by Houxiang et al.,35 also showed that free Hb and percentage of free to total Hb in storage medium also significantly increased after storage as adenosine triphosphate and 2,3-difosfoglicerato levels were significantly decreased compared to fresh RBCs

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