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The antitumor effects of CNSI-Fe have been carried out in a first-in-human phase I clinical trial of dose escalation in subjects with advanced solid tumors in China (NCT07433283)

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Proc Natl Acad Sci USA 108: 74377442, 2011

The formation of these inactive aggregates represents a major bottleneck, influenced by factors ranging from cultivation conditions (e.g., temperature, pH, ionic strength) to intrinsic properties of the target protein itself, such as a high proportion of hydrophobic residues, the presence of intrinsically disordered regions, complex multidomain architectures, or the requirement for specific eukaryotic chaperones or post-translational modifications absent in the prokaryotic host [2, 3]

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