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Al-Hakeim, H

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3.1 Positive control The nucleotide sequence analysis of o riC revealed the presence of a Duplex Unwinding Element (DUE) containing three sets of AT-rich 13mers (which are characterized by a low melting temperature, a propensity to breathe, a high torsional pliability and a reduced stacking energy) in the left extremity of oriC , followed by a DnaAR1 box, an IHF (Host Integration Factor) binding site, a FIS (Factor for Inversion Stimulation) binding site, and high affinity (R1, R2, and R4) and low affinity (r2, l1, l2, c3, c2, l3, and c1) DnaA binding sites