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Description
The tissue underwent graded dehydration by sequential immersion in 30%, 50%, 70%, 90%, 100%, and 100% ethanol, each for 1 h

After weight loss, the ECM is in a compromised state
It's a critical, non-negotiable element of valid research

[DOI] [PMC free article] [PubMed] [Google Scholar] 16.Nwosu B.U

Write the reconstitution date, concentration, and vial contents on a small label or directly on the vial
