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Fractions containing the protein were further purified using a second 1 ml HisTrap TM HP column and eluted with 200 mM imidazole in elution buffer (50 mM HEPES, 500 mM NaCl and 0.05% Sarkosyl)
Detailed analysis reveals interconnected regulatory circuits - from p21Ras activation to downstream metabolic effectors
Liu Y, Zhuang H, Cao F, Li J, Guo Y, Zhang J, et al
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Lenses that are not FDA-approved are also more prone to contamination and can cause infection of the eye with possible permanent visual problems
