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As seen in the figure, all these components eluted from the column in the form of a single symmetrical peak

total cholesterol was measured with CHO2I: ACN 8798 and CHO2A: ACN 8433 in vitro tests

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Then you fight through it, and the next day you wake up, you're like, I'm okay

Then, the well plates were washed with 1 ml of 0.9% NaCl, and the cells were lysed with 1 ml of chloroform:MeOH:H 2 O (1:3:1) and centrifuged at 15,000 g for 5 min at 4 C