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However, it has been suggested that drinking frequency might be more important than the quantity consumed on each occasion,23 as well as the quality of alcohol,24 where Gronbaek et al
ELISA IL8 ELISA was performed with a human IL8 ELISA kit (BioLegend, #431504), according to the manufacturers instructions

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The completely dissolved extract, ensured by vortexing and sonication, was filtered through a 0.22-m organic membrane and then transferred to a reduced volume liquid chromatography vial

10.1002/ajmg.b.30366 143
