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Cells were grown in DMEM/F12 supplemented with 10% fetal bovine serum, 100 g/mL penicillin/streptomycin/gentamicin (Invitrogen, Gibco) and in the condition of 95% air and 5% CO 2 humidified atmosphere at 37 C

Grown cells on solid media were inoculated into 5 mL of the liquid SD medium and aerobically grown at 30 C with agitation (200 rpm) for 24 h

The assay procedure began by adding rat liver tissue samples and TNF- standards to monoclonal antibody-precoated 96-well plates

[DOI] [PMC free article] [PubMed] [Google Scholar] 75.Ciebiera M., Ali M., Zgliczynska M., Skrzypczak M., Al-Hendy A

Air pollution and the risk of Parkinsons disease: a review