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Indeed, small peritoneal macrophages maintain peritoneal ACh levels through choline acetyltransferase expression driven by MyD88 pathway activation

The carnitine shuttle has a role in providing acetyl-CoA groups for acetylcholine synthesis (Figure 3), and in buffering the level of free coenzyme A in the cytosol which can inhibit acetylcholine synthesis via choline acetyltransferase [101]

CSF contains very low metabolite concentrations but contributes to the water signal from the region of interest, so it is necessary to correct the metabolite for this apparent dilution effect

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