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(b) Parameter sensitivity analysis of VCAT on glutathione (GSH) in the integrated oxidative stress and C1 metabolism model

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After three washes with PBS, a goat anti-rabbit Alexa Fluor 568 conjugated secondary antibody diluted 1:600 in GSDB was incubated for 1 h at room temperature followed by three washes with PBS

The same study observed that, in human neuroectodermal cells, folate deficiency was associated with the disrupted recruitment of histones and DNA repair machinery to the required neural tube closure genes, leading to the accumulation of rare genetic variants during neural tube closure, which contributed to the onset of NTDs [258]
