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Compounding this issue is the lack of validated pharmacodynamic biomarkers that directly link OSC exposure to antimicrobial efficacy in humans

USA 104 , 1015810163 (2007)

The dried metabolites were reconstituted in 60% acetonitrile followed by overtaxing for 30 s and then centrifugation for 30 min at 20,000 g at 4 C

The eluted protein was then buffer-exchanged with dialysis buffer (20 mM TrisHCl (pH 8.0) and 200 mM NaCl) and digested with TEV protease at 4 C overnight, to remove the Trx-His 6 -tag

Keeping up with quarterly NCCI and MUE updates is exactly the kind of ongoing compliance work that in-house billing teams don't have capacity for