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The HPLC was set at a flow rate of 0.25 ml min 1 with 99% buffer A from 0 to 3 min, a linear gradient to 99% buffer A/1% buffer B (100% methanol) from 3 to 20 min, 80% buffer A/20% buffer B from 20 to 21 min, a linear gradient to 30% buffer A/70% buffer B from 21 to 28 min at 0.35 ml min 1 , 99% buffer A/1% buffer B from 28 to 31 min and a linear gradient to 99% buffer A from 31 to 37 min at 0.25 ml min 1

The efficacy of both combination therapies and individual herbal treatments has been extensively researched

doi: 10.1586/14789450.5.2.157

Tissue concentrations matter more than serum concentrations, and tissue retention is determined by matrix binding kinetics, not continuous infusion

No significant differences in cell viability between mouse embryonic carcinoma cells (CL6) treated with PEI max and those with PEI max-nanoparticles after 48 h of PEI max or PEI max-nanoparticle exposure 27