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The cells were then scraped on ice, transferred to pre-chilled microcentrifuge tubes, centrifuged at 15,000 g for 20 min and the supernatants were collected

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10.1016/j.ccell.2015.12.004 [DOI] [PMC free article] [PubMed] [Google Scholar] Hayes J.D., Chowdhry S., Dinkova-Kostova A.T., and Sutherland C