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JAMA 298, 4960
doi: 10.1016/J.METABOL.2018.11.001
The supernatant was filtered and applied to a Ni-NTA (first Ni-NTA) column (GE Healthcare) equilibrated with binding buffer A containing 20 mM HEPES pH 7.0, 600 mM NaCl, 10% glycerol, 30 mM imidazole, 2 mM -ME
CAS 42464-96-0)
