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The cell viability was assessed by Hoechst staining of the nucleus (see Methods) and hence assessment of total cell numbers after 72 h of treatment, which is a good proxy for cell survival and cell growth upon prolonged compound exposure

Second, larger vials (10-20 mg) generally get the same 2 mL to keep concentration high enough that GLP-1 weekly doses don't require uncomfortably large injection volumes

Betaine (Oral Route) Proper Use Mayo Clinic

Exp Biol Med (Maywood) 238(4):360369

Never inject a solution that looks different from when you first reconstituted it
