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2.5 Affinity isolation of HPX binding proteins We used well-controlled affinity chromatography to isolate putative hemopexin receptors (HPXRs) from whole cell extracts (100,000 x g isolates in HEPES buffered pH 7.4 Triton X-100 0.5%, 150 mM NaCl containing phosphatase and protease inhibitors, Millipore Sigma) of non-hepatic human promyelocytic HL-60, HepG2 cells, and freshly plated primary human hepatocytes

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Krka, Slovenia)), we induced abdominal compartment syndrome by intraperitoneal insufflation of ordinary air controlled by a manual and digital manometer with a data logger connected to a computer (DD890, Dostmann Electronic GmbH, Germany) and maintained high abdominal pressure at 25 mmHg for 120 min before sacrifice, with a pressure measurement interval of 1 s

How to eat it: Add sliced avocado to your morning toast, mash it into guacamole, or throw it into smoothies for a creamy texture and a nutrient punch

doi: 10.3390/antiox12101812 146 SebastianiGNavarro-TapiaEAlmeida-ToledanoLSerra-DelgadoMPaltrinieriALGarca-Algaret al