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As for the thyroid hormone and its receptor, there appears to be no reported direct interaction with the POMC promoter, although POMC -/- animals are known to display primary hyperthyroidism (34)

Briefly, a single oocyte was loaded in a PCR tube with 10 l lysis buffer (proteinase K added) and incubated at 55 C for 2 hours and 95 C for 10 min, and then the sample was used directly for qPCR analysis

Moreover, in comparison with the placebo, a significant improvement in cognitive functions and other neurological parameters was observed in the treatment group, warranting further investigation in larger cohorts