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Adding TB-500 increases both cost and complexity, so it makes sense to reserve the stack for more challenging cases
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The inter-assay variability range has now been added into the text (Results, line 4): This assay has an inter-assay coefficient of variability of 8.8 % as calculated with a single serum sample measured on 18 different plates (24.11 2.12 ng/ml (SD)
Another fact to note is that, along with vitamin B12, almost every diet requires augmentation of some kind
