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[DOI] [PMC free article] [PubMed] [Google Scholar] (182).Lamb J

Importantly, both interventions increase both lifespan and healthspan without apparent major fitness trade-offs, in contrast to those previously reported in long-lived mutants that do slow the rate of ageing 39, 40
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Lysed bone marrow cells were stained with biotin-conjugated lineage marker antibody cocktail:CD3 monoclonal antibody (ThermoFisher Scientific, #13-0037-82, 1:200 dilution), CD4 monoclonal antibody (ThermoFisher Scientific, #13-0041-85, 1:100 dilution),CD11b monoclonal antibody (ThermoFisher Scientific, #13-0112-85, 1:100 dilution), CD5 monoclonal antibody (ThermoFisher Scientific, #13-0051-85, 1:200 dilution), CD8 monoclonal antibody (ThermoFisher Scientific, #13-0081-85, 1:200 dilution), CD45R (B220) monoclonal antibody (ThermoFisher Scientific, #13-0452-85, 1:200 dilution), Ly-6G monoclonal (ThermoFisher Scientific, #13-5931-85, 1:200 dilution),TER-119 monoclonal antibody (ThermoFisher Scientific, #13-5921-82, #1:200 dilution), at 4 C for 30 min
