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In service of the immune response, histamine can also instigate a number of other reactions: Contracting smooth muscle tissues Lowering blood pressure Accelerating the heart rate Initiating chemical communication between nerve cells

Wang, Y., Qu, W

However, whereas neutralizing antibodies are constrained to recognizing limited epitopes involved in binding to and fusion with host cells, up to 95% of total spike-specific antibodies are non-neutralizing and can bind to epitopes spread across the spike protein 107,108

doi:10.1016/j.arr.2016.08.006

The reaction time was defined by placing the mouse on the hot plate, and the time was recorded when the animal showed hind paw licking or jumping, and the 45 sec period was observed to avoid tissue damage