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Costimulation serves as a mechanism to modulate the strength of the TCR signal and promote higher gene expression of immunomodulatory cytokines like interleukin-2, as well as other factors that facilitate T cell proliferation and differentiation ( A key mediator of T cell signaling is the divalent cation calcium (Ca 2+ )

doi: 10.1097/01.ASN.0000077407.90309.65

Since application of big amount of data from EEM measurements might lead to model overfitting [38], additional parameters of RMSE (Root Mean Square Error of Calibration) and RMSEP (Root Mean Square Error of Prediction) were also taken into account to better access and compare generalization capabilities of the obtained models (Table 1)

10.1016/j.ydbio.2012.12.001 Dev
