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Briefly, primary cells were washed with PBS, lysed in 100 l of 0.4 N perchloric acid (PCA) for 30 min at 4 C, and centrifuged, and the supernatants were neutralized with four volumes of 0.1 M NaH 2 PO 4 and 5 mM EDTA, pH 7.5

N., Uchakin, P

elegans strains were maintained on standard NGM plates seeded with OP50 E.coli at 20 Celsius degree

10.7554/eLife.31274 15 BroekmansF

[DOI] [PMC free article] [PubMed] [Google Scholar] 81.Haenisch B, Bnisch H
