glutathione s-transferase gst pull-down assay Pull down Glutathione S-transferase (GST) pull-down assay
Description
Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

Add it to the water phase and keep processing temperatures below 40 C (104F)

Inflammatory markers, specifically interleukin-6, were also significantly lower in losartan-treated groups compared to the ethanol group

If you are a drug-tested athlete at any level, do not take BPC 157 LPT, as it may be on the banned substance list for your sport or organization
FDA APPROVED
