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After euthanizing the mice on day 12, periwound skin tissue was collected from different groups for follow-up experiments, as detailed in the subsequent section

Gene expression analysis with RTqPCR Gene expression was analyzed via reverse transcriptase quantitative polymerase chain reaction (RTqPCR) using TaqMan probes (Life Technologies) or SSO Universal Probes Master Mix (Bio-Rad)

Functions of the cardiovascular system The exact outline of the circulatory system's functions is well known to everyone, so its presentation will be typically in the form of a reminder

A microdose is any weekly dose below the 2.5 mg label starter, usually 0.5-2 mg weekly

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