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[DOI] [PubMed] [Google Scholar] 101.Guo G, Gong K, Wohlfeld B, Hatanpaa KJ, Zhao D, Habib AA

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The thermocycling protocol was the following: initial denaturation at 95C for 10 minutes, followed by 40 cycles of 15 seconds at 95C, 45 seconds at either 58C or 60C (depending on primer specificity), and 30 seconds at 72C

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