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doi: 10.1097/wnr.0000000000000254 120

Immunoprecipitation Cells between 48 and 72 h of transfection were collected, pre-cooled, washed with PBS, lysed using NTEN buffer (20 mM Tris-HCl (pH 7.5), 10% glycerol, 150 mM NaCl, 0.5% NP40, 1 mM PMSF, 1 g/mL leupeptin, and 1 g/mL aprotinin), and sonicated of 10 s on/20 s off for three cycles by Qsonica sonicator (Q800R3)

Now one week out from day 8

On the other hand, when we introduced random modifications to enzyme and transporter concentrations and their kinetic parameters, some numeric solutions failed or diverged far from the steady state at rest