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When neurofibromin is absent or non-functional, RAS remains constitutively active, driving downstream MEK/ERK pathway hyperactivation and uncontrolled cell proliferation

Preferred sites: Abdomen (avoid 2 inches around navel) Front or outer thigh Upper arm (posterior) Technique: Clean injection site with alcohol swab Let alcohol dry completely Pinch fold of skin Insert needle at 45-90 degree angle Inject slowly over 5-10 seconds Release skin, withdraw needle Light pressure if any bleeding Rotate sites to prevent tissue irritation

Moreover, between 11 and 19% of patients who survive have severe sequelae, such as hearing loss, limb amputation, and neurological complications (Olbrich et al., 2018

doi: 10.1038/s41582-020-0370-2, PMID: [DOI] [PubMed] [Google Scholar] 32.Morris HR, Spillantini MG, Sue CM, Williams-Gray CH

GPX1-4 and GPX6 are neutral to selenocysteine, so they can mediate redox reactions by interacting with GSH (Figures 1, 8)