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In all conditions tested, band 3 levels were not significantly different with respect to those determined in untreated RBCs

A newer, versatile approach for measuring cAMP after Gs- or Gi-mediated signaling is the cAMP difference detector in situ (cADDis) assay (schematic representation, Figure 5)

The supernatant was discarded and pre-cooled fixative (2.5% glutaraldehyde) at 4 C was slowly added along the wall of the tube, before refrigeration at 4 C overnight prior to subsequent processing