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Once inside, aminoglycosides accumulate within hair cells to concentrations that persist for months owing to high-affinity binding to phosphatidylinositol 4,5-bisphosphate ( Mitochondrial dysfunction Aminoglycosides preferentially localize to mitochondria by binding to the 12S ribosomal RNA, which is encoded by the mitochondrially encoded gene MT-RNR1

TafazoliS.Mechanisms of drug-induced oxidative stress in the hepatocyte inflammation model,Doctor of Philosophy, Department of Pharmaceutical Sciences, University of Toronto,2008 27

The ROS level was measured using DCFDA/H2DCFDA cellular ROS assay kit (Abcam, AB113851)

doi: 10.1111/1471-0307.12759 198

For a full breakdown of how these peptides interact biologically, see How the Wolverine Stack Works (Mechanisms of BPC-157 & TB-500 Synergy)