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Bock M, Steffen F, Zipp F, Bittner S

All primary antibody incubations (1:1000 in TBST, overnight at 4 C) were followed by incubation with a secondary HRP-conjugated antibody (1:5000 in TBST, 1 h at room temperature), and the signals were visualized using a chemiluminescent HRP substrate on a chemiluminescence imaging system (CHAMPCHEMI TM Chemiluminescent Imaging and Analysis System)

This localized depletion of the brain's master antioxidant is accompanied by abnormally high levels of ventricular lactate and free radicals, indicating that the brain is suffering from severe oxidative stress and impaired cellular energy metabolism

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[16] Ilan Y